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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
04/10/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, I.; ANTUNES, G. R.; PAIVA, D. de S.; LANGE, C. C.; GUIMARÃES, S. E. F.; MARTINS, M. F. |
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Afiliação: |
ISABELA FONSECA, UFV; GUSTAVO RESENDE ANTUNES, UFJF; DAISYLÉA DE SOUZA PAIVA, UFJF; CARLA CHRISTINE LANGE, CNPGL; SIMONE ELISA FACIONI GUIMARÃES, UFV; MARTA FONSECA MARTINS, CNPGL. |
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Título: |
Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 10, n. 3, p. 1295-1303, 2011. |
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DOI: |
https://doi.org/10.4238/vol10-3gmr1096 |
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Idioma: |
Inglês |
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Conteúdo: |
Among the potential public health problems of animal production, infectious-contagious diseases stand out. Mastitis is among the main diseases affecting dairy cattle. One of the most promising options to reduce the problems caused by this disease, besides proper sanitary and management practices, is selective breeding of resistant animals. To shed light on the immune response mechanisms involved in the resistance/susceptibility phenotype to this disease, we quantified the relative expression of the genes IL-2, IL-6, IL-8, IL-12, IFN-γ, TNF-α, TLR-2, SEMA5A, and FEZL in cells of crossbreed dairy cows, divided into two groups, one healthy and the other suffering from clinical mastitis. Total RNA was extracted from the cells in the milk from the animals in each group (with and without clinical mastitis). Gene expression was determined using the real-time PCR method. The levels of gene expression were compared, and the cows with mastitis were found to express 2.5 times more TLR-2 than those free of mastitis (P < 0.05). There were no significant differences in the expression of the other genes. |
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Palavras-Chave: |
Real-time PCR; Resistance to mastitis; Toll-like receptor 2 gene. |
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Thesaurus Nal: |
immune response. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/54216/1/Differential-expression-of-genes-during.pdf
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Marc: |
LEADER 01871naa a2200241 a 4500
001 1902309
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4238/vol10-3gmr1096$2DOI
100 1 $aFONSECA, I.
245 $aDifferential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows.$h[electronic resource]
260 $c2011
520 $aAmong the potential public health problems of animal production, infectious-contagious diseases stand out. Mastitis is among the main diseases affecting dairy cattle. One of the most promising options to reduce the problems caused by this disease, besides proper sanitary and management practices, is selective breeding of resistant animals. To shed light on the immune response mechanisms involved in the resistance/susceptibility phenotype to this disease, we quantified the relative expression of the genes IL-2, IL-6, IL-8, IL-12, IFN-γ, TNF-α, TLR-2, SEMA5A, and FEZL in cells of crossbreed dairy cows, divided into two groups, one healthy and the other suffering from clinical mastitis. Total RNA was extracted from the cells in the milk from the animals in each group (with and without clinical mastitis). Gene expression was determined using the real-time PCR method. The levels of gene expression were compared, and the cows with mastitis were found to express 2.5 times more TLR-2 than those free of mastitis (P < 0.05). There were no significant differences in the expression of the other genes.
650 $aimmune response
653 $aReal-time PCR
653 $aResistance to mastitis
653 $aToll-like receptor 2 gene
700 1 $aANTUNES, G. R.
700 1 $aPAIVA, D. de S.
700 1 $aLANGE, C. C.
700 1 $aGUIMARÃES, S. E. F.
700 1 $aMARTINS, M. F.
773 $tGenetics and Molecular Research$gv. 10, n. 3, p. 1295-1303, 2011.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
16/02/2011 |
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Data da última atualização: |
22/11/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
JORGE, E. C.; MELO, C. M. R. de; ROSÁRIO, M. F. do; ROSSI, J. R. S.; LEDUR, M. C.; MOURA, A. S. A. M. T.; COUTINHO, L. L. |
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Afiliação: |
ERIKA CRISTINA JORGE, ESALQ; CLÁUDIO MANOEL RODRIGUES DE MELO, UFSC; MILLOR FERNANDES DO ROSÁRIO, ESALQ; ESALQ; MONICA CORREA LEDUR, CNPSA; ANA SILVIA ALVES MEIRA TAVARES MOURA, UNESP; LUIZ LEHMANN COUTINHO, ESALQ. |
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Título: |
Chicken skeletal muscle-associated macroarray for gene discovery. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v.9, n.1, p.188-207, 2010. Disponível em: . Acesso em: 16 fev. 2011. |
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Idioma: |
Inglês |
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Notas: |
Projeto/Plano de Ação: 01.02.102.10-10. |
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Thesagro: |
Genética. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/27346/1/jorge-gmr712.pdf
https://www.funpecrp.com.br/gmr2008b/issue.asp?volume=586&capitulo=547
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Marc: |
LEADER 00682naa a2200205 a 4500
001 1877426
005 2013-11-22
008 2010 bl uuuu u00u1 u #d
100 1 $aJORGE, E. C.
245 $aChicken skeletal muscle-associated macroarray for gene discovery.
260 $c2010
500 $aProjeto/Plano de Ação: 01.02.102.10-10.
650 $aGenética
700 1 $aMELO, C. M. R. de
700 1 $aROSÁRIO, M. F. do
700 1 $aROSSI, J. R. S.
700 1 $aLEDUR, M. C.
700 1 $aMOURA, A. S. A. M. T.
700 1 $aCOUTINHO, L. L.
773 $tGenetics and Molecular Research$gv.9, n.1, p.188-207, 2010. Disponível em: <www.funpecrp.com.br>. Acesso em: 16 fev. 2011.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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